The role of its anti-tumor of the flavonoids in the tea flower

In order to promote the development of the tea industry, the flavonoids in the tea flowers were extracted and its anti-tumor effect was investigated. First, MCF-7 was dropped into 96 well plates and cultured at 5% CO 2 , 37℃ in 24 hours. 25μL medium 1640, 25μL distilled water, 25μL 0.5c flavonoids extract solution from the tea flower was respectively added to MTT Assay. Next, MCF-7 was dropped into 6 well plates and cultured to the full well at 5% CO 2 , 37℃ . 170μL medium 1640, 170μL distilled water, 170μL 0.5c flavonoids crude extract solution was respectively added to Scratch Assay. The results showed that in MTT Assay, the higher the flavonoids extract solution concentration was, the lower the cell survival rate was, that was to say, the cell survival rate was 0.5c>0.25c>0.125c. In Scratch Assay, MCF-7 cell scratch space at the blank control group (medium 1640) and negative control group (distilled water ) gradually shortened and varied greatly , but the 0.5c flavonoids extract solution′ s was almost no change. The study suggested that the flavonoids in the tea flower could strongly inhibit the MCF-7 growth.