Study on the dynamic changes of microorganisms in 2 different processing of slaughter lamb during storage by PCR-DGGE

16S rDNA DGGE (denaturing gradient gel electrophoresis) finger printing and phylogenetic analysis were used to reveal the dynamic changes of predominant bacterial in electrical stimulation and slung-load of processing for slaughter lamb during storage at 4℃. The analysis of the dynamic bacterial community directly from the DGGE profiles showed various types of bacterial communities in electrical stimulation group in the initial storage period, indicating that the contamination microorganisms were diversitied. But only few types of bacteria survived and became the predominant bacteria eventually. Compared with electrical stimulation group, the slung-load group had fewer bacterial communities. The DNA sequences of main bands were analyzed and the main bacterial of lamb was found to be Psychrobacter sp, Staphylococcus sp, Klebsiella sp, Pseudomonas sp, Stenotrophomonas sp, Enterobacter sp, Lactobacillus sp and Brochothrix thermosphacta. During the early storage, the two groups microbial flora was insignificant. But after 10d, the Staphylococcus sp. was the predominant bacteria in hanging group. The predominant bacteria of electrical stimulation group were Pseudomonas sp and Psychrobacter sp.