Science and Technology of Food Industry

ISSN 1002-0306
CN   11-1759/TS
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Fermentation optimization of alkaline lipase produced by the pseudomonas BL11[J]. Science and Technology of Food Industry, 2013, (09): 160-163. DOI: 10.13386/j.issn1002-0306.2013.09.032
Citation: Fermentation optimization of alkaline lipase produced by the pseudomonas BL11[J]. Science and Technology of Food Industry, 2013, (09): 160-163. DOI: 10.13386/j.issn1002-0306.2013.09.032
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Fermentation optimization of alkaline lipase produced by the pseudomonas BL11

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  • The optimal culture medium and culture conditions of alkiline lipase produced by the pseudomonas BL11 was studied by single-factor experiment and uniform design.The optimized medium was maltose 2.5%, peptone 3.0%, soybean oil 0.5%, K2HPO4 0.2% and the optimized culture conditions was initial pH7.5, medium volume 40mL/250mL flask, culture temperature 33℃, rotatiaon rate 180r/min.The highest enzyme activity was 223U/mL after 60h.
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