Study on inhibitory on tyrosinase and antioxidant activity of polyphenols from peanut testa

The optimum technology of extracting total polyphenols of peanut testa by flash-extraction was established using single factor experiment and orthogonal test.The inhibitory effect on tyrosinase and scavenging action on DPPH· of polyphenols were studied.The optimum extraction conditions were as follows:ethanol concentration of 80%, material-to-liquid ratio of 1∶25 (g/mL) , 90V voltage, three times extraction, each for 2min.Results indicated that under these conditions the extraction yield of total polyphenols was 96.94%.The mass concentration of polyphenols in the extract needed for inhibitory effect on tyrosinase to 50% (IC50) was 0.451mg/mL.And the highest inhibition rate was 89.65%, the scavenging action of polyphenols to DPPH·was obviously higher than VC, a little weaker than gallic acid.