Study on the liquefied and saccharified craft for the dual-enzyme hydrolysis method of making injection-glucose with broken rice

Objective:To study on the liquefied and saccharified craft for the dual-enzyme hydrolysis method of making injection-glucose with broken rice, and to explore the best process conditions suitable for the preparation of the liquefaction and saccharification. Methods:Used double enzyme method, and mathematical model of Michaelis-Menten equation. Chose the value of DE, the protein content, and the light transmission rate and other indicators. Results:The best grinding broken head was 60⁸0 mesh. Optimum amount of liquid enzyme 160U·g-1. Liquefaction process for the best pieces rice was to 60 mesh, mixing the concentration was 25% to 30%, liquefaction time was 30min, CaCl2 amount was 0.3%, pH was 6.0, temperature was 90⁹5℃, Hong Ying Xiang enzyme dosage was 160U/g. Optimum saccharification Hong Ying Xiang glucoamylase for the amount was 150U/g dry matter, pullulanase dosage was 0.25U/g dry matter, saccharification time was 24h, pH of 4.2, the temperature was 60℃. Conclusion:Through this study, the best craft for the liquefaction and saccharification process conditions with the broken rice was solved, and the foundation of making glucose injection with broken rice was set.