HPLC fingerprint and its chemical pattern recognition on bramble juice in Kashgar

Objective:The fingerprint of bramble juice in Kashgar was established by use of HPLC, using the common peak as the evaluation index, and combined with the similarity analysis, the quality of the juice was evaluated. Methods:Agilent HC-C18 column (4.6×250mm, 5μm) was used with gradient elution of methanol (B) -0.5% acetic acid (A) as mobile phase. Gradient elution:0～8min, 10%～20% B, 8～15min, 20%～35% B, 15～25min, 35%～50% B, 25～40min, 50%～80% B. The detection wavelength was 275nm, the flow rate was 0.8mL/min and the column temperature was 30℃. Results:The fingerprint of bramble juice was established by using sample chromatography of 12 batches and 13 common fingerprint peaks were found. There was little difference in the similarity between sample chromatograms and the standard spectrum (similarity above 0.968) , which proved that bramble juice quality of 12 batches was stable. Moreover, it was more different from counterfeit. The fingerprints were obtained and were handled by hierarchical clustering analysis and principal component analysis. Conclusion:The result showed that the method was convenient for differentiation of counterfeit and applicable to quality evaluation of bramble juice in Kashgar.