Enzymatic assisted extraction of polyphenolics from red pine hull

In the present study, the extraction process, based on ethanol extraction of polyphenolics, was modified by adding cellulase in order to increase the extraction rate of polyphenolics from red pine hulls.Ethanol concentration, enzymatic hydrolysis time, materials to water rate, enzymatic hydrolysis temperature, enzyme additive amount, and pH were chosen as six factors, and polyphenolic extraction rate was selected as the response factor.The optimization of polyphenolics extraction rate was performed by employing Small Composite (Hartley Method) and Response Surface Methodology design.The polynomial regression equation was well established.The optimized parameters were as follows:ethanol concentration 61 %, enzymatic hydrolysis time 2h, water to materials rate 36:1, enzymatic hydrolysis temperature 62℃, enzyme additive amount 106U/g, and pH4.6.The extraction rate was 8.93mg/g under optimized conditions.Compared with the nonenzymatic ethanol extraction process, the extraction rate was increased by 0.97mg/g with the assistance of cellulase.