Anticancer effect of different concentrations of Ilex kudingcha C.J.Tseng in TCA8113 human tongue squamous carcinoma cells in vitro

One Ilex kudingcha C.J.Tseng product was sold for the evaluation of in vitro anticancer effect in TCA8113 human tongue squamous carcinoma cells.In MTT assay, apoptosis analysis by DAPI staining and RT-PCR, Western blot gene expressions were employed to check their anticancer effect.Using MTT assay to check the in vitro cancer cell growth inhibitory effect, at the concentration of 200μg/mL, Ilex kudingcha C.J.Tseng (75% inhibition rates) showed the best inhibitory effect on the growth of TCA8113 human tongue squamous carcinoma cells.DAPI staining showed that 200μg/mL of Ilex kudingcha C.J.Tseng induced apoptosis on TCA8113 cells.Ilex kudingcha C.J.Tseng (200μg/mL) significantly induced apoptosis, up regulated mRNA and protein expressions of Bax, caspase-3 and caspase-9, and down-regulated Bcl-2 expression, compared with 100 and 50μg/mL of Ilex kudingcha C.J.Tseng.These results suggested that Ilex kudingcha C.J.Tseng showed good cancer preventive effect.