Physical and chemical properties of collagen from the skin of channel catfish

The acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from skin of channel catfish by the method of acid and acid-pepsin, and their physical and chemical characterization were analysed.The study indicated that the ASC had high purity of 93.11% and PSC of 93.46%, the analysis of UV spectra of ASC and PSC showed that the maximum absorption was obtained at 233nm.Both ASC and PSC consisted of two different α-chains (α 1) 2 α 2 were characterized as type I.Fourier transform infrared (FTIR) spectra of both ASC and PSC were almost similar with whole triple-helical structure.Transition temperature of ASC and PSC was 34.2℃ and 33.9℃.