Extraction of polyphenlols from Pinus Sylvestris L.and comparison of extraction method

In order to search an effective extraction of polyphenols from Pinus Sylvestris L., the solvent extraction, ultrasonic-assisted extraction and ultrasonic-compound enzymatic assisted extraction were explored.The results indicated that the optimum condition of solvent extraction were as follows:time was 4h, ethanol concentration was 60%, temperature was 60℃, ratio of material to solvent was 1:25.The optimal conditions to obtain the highest yield of polyphenols for ultrasonic extraction were determined as follows:ultrasonic time was 2.5h, ultrasonic power was 300W, ultrasonic temperature was 60℃, pH value was 3.0.The best technical conditions for ultrasonic-compound enzymatic assisted extraction were as follows:enzymatic hydrolysis time was 40min, temperature was 45℃, enzyme concentration was 4%, pH value was 4.0.The polyphenol yields of the solvent extraction, ultrasonic-assisted extraction and ultrasonic-compound enzymatic assisted extraction method were 12.56, 23.01, 30.12mg/g respectively.Ultrasonic-compound enzymatic assisted extraction method not only greatly shortened processing time, but also produced higher yield of polyphenols components.The results provided an industrial reference for developing new extraction processes of polyphenols components from Pinus Sylvestris L.