Preliminary optimal ion-exchange technology of saccharification liquid made from broken rice for preparing glucose injection by response surface methodology

To optimize the ion-exchange technology conditions of saccharification liquid from broken rice by central composite design and response surface methodology. Taking resin loading quantity, pH and elution speed as investigation factors, and the elution efficiency as effect size.The multiple linear regression and binomial equation were fitted respectively, and response surface methodology was adopted to optimize the technology, predict and analyze the possible result. The optimum technology for ion- exchange conditions of saccharification liquid from broken rice was like this:10g cation exchange resin ( pH 4.0) and 10g anion exchange resin ( pH 10.0) were added into two chromatographic columns ( 10mm × 20cm ) severally, eluted at the speed of 10d /min under room temperature.The above-mentioned experiment proved the ion-exchange technology was simple, and practicable, and it could provide experimental basis for ion-exchange technology research on further preparation of the glucose injection.