Optimization of ultrasonic extraction technique of SOD from horse blood by response surface methodology

doi:10.13386/j.issn1002-0306.2013.02.070

Optimization of ultrasonic extraction technique of SOD from horse blood by response surface methodology[J]. Science and Technology of Food Industry, 2013, (02): 249-251. DOI: 10.13386/j.issn1002-0306.2013.02.070

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Optimization of ultrasonic extraction technique of SOD from horse blood by response surface methodology

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Based on single factor experiment, three main factors were creened out, including the ultrasonic time, ultrasonic power, ultrasonic temperature. Optimization of these factors by using response surface methodology was studied. Results of response surface analysis showed that the optimum ultrasonic extraction conditions of SOD were as follows : ultrasonic time 15min , ultrasonic power 220W , the ultrasonic temperature 31 ℃ . Under these conditions, specific activity of SOD was 77.5U/mg and which was in good agreement with the predicted value.

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Optimization of ultrasonic extraction technique of SOD from horse blood by response surface methodology

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