Optimization of Mortierella Isabellina culture medium conditions in fermentation of arachidonic acid by response surface methodology

Fermentative production arachidonic acid of Mortierella isabellina using waste water from maize paste was investigated to reduce the cost, adding waste water from maize paste into the culture medium had no obvious effect on the fermentation. On the basis of single factor tests, response surface analysis was designed by Design Expert, and the glucose concentration, different quality of waste water from maize paste and pH were investigated. The results showed that the optimal culture medium conditions were the glucose concentration was 90g/L, the maize paste waste water was 25% in the culture medium and pH was 6. Under this condition, the yield of arachidonic acid was 3.11g/L.