A novel method for determining the coating efficiency of carboxymethyl chitosan coated liposomes containing coix seed oil

Ammonium thiocyanate method was applied to determine coating efficiency for carboxymethyl chitosan coated liposomes. The result showed that the wavelength for determination of phospholipid was at 470nm . At the concentration range of 0 . 01 ~ 0 . 1mg / mL , the content of phospholipid showed good linear relationship with the absorbance. Before determination, the uncoated liposomes were isolated from the coated ones by centrifugation operated at 10000r/min for 15min and broken by alcohol-ultrasound method with 0.8mL of ethanol and ultrasonication for 15min. Through recovery, precision, and reproducible experiments proved that the method was feasible.