Breeding of high β-galactosidase producing Aspergillus Oryzae by mutation with UV and LiCl

doi:10.13386/j.issn1002-0306.2013.01.035

Breeding of high β-galactosidase producing Aspergillus Oryzae by mutation with UV and LiCl[J]. Science and Technology of Food Industry, 2013, (01): 155-159. DOI: 10.13386/j.issn1002-0306.2013.01.035

Citation:

Breeding of high β-galactosidase producing Aspergillus Oryzae by mutation with UV and LiCl[J]. Science and Technology of Food Industry, 2013, (01): 155-159. DOI: 10.13386/j.issn1002-0306.2013.01.035

Citation:

Breeding of high β-galactosidase producing Aspergillus Oryzae by mutation with UV and LiCl[J]. Science and Technology of Food Industry, 2013, (01): 155-159. DOI: 10.13386/j.issn1002-0306.2013.01.035

Breeding of high β-galactosidase producing Aspergillus Oryzae by mutation with UV and LiCl

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In order to breed high-yield β-Galactosidase producing strain, Aspergillus Oryzae (ATCC20423) was used as the original strain and mutagenized with Ultraviolet (UV) and UV+lithium chloride (LiCl) , respectively.After mutagenized by UV, the β-Galactosidase activity of UV-15-20 reached 114.08U/mL, which revealed a 49.22% increase compared with the original strain.A mutant UV-LiCl-38 with high β-Galactosidase activity (121.42U/mL) was obtained by use of UV and LiCl, the β-galactosidase activity of this mutant was 58.82% higher than that by the wild-type.UV-15-20 and UV-LiCl-38 had good hereditary stability after several generations.

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Breeding of high β-galactosidase producing Aspergillus Oryzae by mutation with UV and LiCl

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