Optimization of culture medium for γ-decalactone production by Yarrowia sp.using Taguchi design

Culture medium for γ-decalacton production by Yarrowia sp.was optimized by using Taguchi design.First, single factor was tested, then orthogonal experiment was designed to carry out using Minitab software, and the result of the mean and SNR of level of each factor were analyzed by using Taguchi design.The result proved that the effect of composite nitrogen was very significant, and (NH4) 2HPO4 was significant.Software predicted the optimal culture medium consisted of 20g/L wheat bran, 7.5g/L yeast extract, 16.5g/L (NH4) 2HPO4, 6g/L KH2PO4 and 0.5g/L MgSO4·7H2O, and GDL yield was 2.49g/L, the SNR was 9.04dB.The γ-decalacton yield of verification experiment was 2.65g/L, and the SNR was 9.68dB.The result showed that the optimal solution achieved the desired effect with good stability.