Optimization of expression conditions of recombinant human-source matrix metalloproteinase 2 hemopexin-like C-terminal domain

To optimize fermentation conditions of Genetically Engineered Escherichia coli (BL21 (DE3) /PET42a-PEX) , Box-Behnken design was used to establish mathematical model based on single-factor tests.The response surface methodology (RSM) analysis result showed that the optimal scale of factors was:Luria-Bertani liquid culture medium pH7.50, bacterial liquid optical density (OD600) 0.60, induce concentration of IPTG 0.60mmol/L, induce time 5.0h.Under these optimized conditions, the yield of PEX was 20.98mg/L.Optimization of expression conditions of genetically engineered Escherichia coli expressing recombinant human-source matrix metalloproteinase 2 hemopexin-like C-terminal domain was studied, and laid the foundation of generation of human recombinant PEX by fermentation.