Meat species authenticity identification by loop-mediated isothermal amplification assay in beef and mutton

doi:10.13386/j.issn1002-0306.2012.22.037

Meat species authenticity identification by loop-mediated isothermal amplification assay in beef and mutton[J]. Science and Technology of Food Industry, 2012, (22): 60-62. DOI: 10.13386/j.issn1002-0306.2012.22.037

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Meat species authenticity identification by loop-mediated isothermal amplification assay in beef and mutton

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A loop-mediated isothermal amplification (Lamp) method was developed for meat ingredients authenticity identification in beef and mutton. A set of universal primer used for Lamp at 63℃ was chosen to differentiate animal gene encoding cytochrome b with high sensitivity. 0.01%～2% pork could be detected in beef and mutton by the method. Besides, the method could be used to detect animal meats processed or unprocessed.

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Science and Technology of Food Industry

Meat species authenticity identification by loop-mediated isothermal amplification assay in beef and mutton

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