Optimization of derivatization conditions for the determination of 1-deoxynojirimycin by response surface methodology

doi:10.13386/j.issn1002-0306.2012.22.004

Optimization of derivatization conditions for the determination of 1-deoxynojirimycin by response surface methodology[J]. Science and Technology of Food Industry, 2012, (22): 63-67. DOI: 10.13386/j.issn1002-0306.2012.22.004

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Optimization of derivatization conditions for the determination of 1-deoxynojirimycin by response surface methodology

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For the purpose of establishing a high performance liquid chromatography method for the determination of 1-deoxynojirimycin (1-DNJ) , the optimal derivatization conditions were studied by response surface methodology. Based on the result of single factor experiments, the Box-Behnken design was used to investigate the effect of derivative time, temperature and the amount of derivative reagent on the recovery of 1-deoxynojirimycin. The derivative conditions were found to be 22min, 29℃ and 0.43mmol/L and the recovery was 99.4%.

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Optimization of derivatization conditions for the determination of 1-deoxynojirimycin by response surface methodology

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