Preparation of broad-specific antibody to fluoquinolone drugs based on lomefloxacin immunogen

doi:10.13386/j.issn1002-0306.2012.20.055

Preparation of broad-specific antibody to fluoquinolone drugs based on lomefloxacin immunogen[J]. Science and Technology of Food Industry, 2012, (20): 156-159. DOI: 10.13386/j.issn1002-0306.2012.20.055

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Preparation of broad-specific antibody to fluoquinolone drugs based on lomefloxacin immunogen

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Using lomefloxacin as hapten, the immunogen was prepared with water soluble carbodiimide (EDC) method, and the coating agent was synthesized with active ester method.The polyclonal antibody was obtained successfully after immunization.Characterized by indirect competitive enzyme linked immunosorbent assay (icELISA) , the antibody exhibited strong broad-specificity, 11 fluoroquinolone drugs could be recognized with a limit of detection lower than 10ng/mL, which could meet the requirement of related international organization.This study would benefit to the immunoassay development for the simultaneous detection of many fluoroquinolone drugs.

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Preparation of broad-specific antibody to fluoquinolone drugs based on lomefloxacin immunogen

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