Study on kinetics of the hydrolysis of Crassostrea gigas oyster protein by compound enzymes

The hydrolysis kinetic model of Crassostrea gigas oyster protein catalyzed with compound enzymes (enzyme activity proportion between flavourzyme and trypsin was 2.3:1) was studied in this paper.The influence of substrate concentrations and enzyme concentrations on the degree of hydrolysis (DH) were discussed by the formol-titration method.Therefore, the kinetic models were established.The relative parameter of the models was determined, the relation between molecular weight, peptide chain long and the enzymolysis time were analyzed.The result of predetermination from the kinetic models was consistent with the practical one, the hydrolysis kinetic model could be applied on the preparation of oyster biological active peptide products.