Study on the extraction conditions and antioxidition of polyphenol from Picea Koraiensis Nakai

The paper utilized solvent to extract polyphenols from Picea Koraiensis Nakai pinecone.The effect of ratio of material to liquid, alcohol concentration, extraction temperature and extraction time on the polyphenol yield was researched by single factor experiment and orthogonal test.At the same time, the ability of total antioxidition, the ability to eliminating OH., DPPH.and O 2.were investigated.The result showed that the optimum extraction condition of polyphenols was as the following:the ration of liquid to material was 25:1, alcohol concentration was 60%, the extraction temperature was 60℃, the extraction time was 3h, the yield of polyphenols extraction from Picea Koraiensis Nakai pinecone was 136.3mg/g.The polyphenol extraction from Picea Koraiensis Nakai pinecone had good eliminating effect on ABTS +., OH., DPPH.and O 2., that was better than V C and BHA.