Ultrasonic assisted in the aqueous enzymatic extraction of camellia seed protein by the comparison of five enzymes and research of optimization of process by the response surface methodology

Considering camellia seed as a raw material, using aqueous enzymatic combination of ultrasonic pretreatment technology extracts camellia seed protein, the effect of five enzymes on the extraction rate of camellia seed protein's was studied.Selecting the optimal ultrasound parameters, based on the orthogonal experiment and using the optimal ultrasound parameters, at 1:6 solids-to-liquid ratio, enzymatic hydrolysis time for 3.5h, 0.02% of the enzyme concentration, the optimum pH and temperature, camellia seed protein's extraction rate follows in descending order:Kemzyme>Viscozyme L>Protex 7L>Alcalase2.4L>Protex 6L.Based on single factor experiment, response surface optimization method was used to determine the optimal condition for protein extraction.The result showed that Kemzyme at 1:5 solids-to-liquid ratio, enzymatic hydrolysis time for 3.24h, enzymatic hydrolysis temperature for 68℃, pH7.28, amount of enzyme 0.048%, optimum value of response surface was 20.42%.