Optimization of heparin extraction process from bovine lung

The bovine lungs as the research material, extracted and separated heparin with the salt solution-resin adsorption and protein to remove impurities precipitation method.Three extraction parameters including material liquid ratio, salt concentration and resin content were optimized using rotating orthogonal combination design principle and the response surface method for process optimization.The results indicated that the best extraction process were material liquid ratio for 1:15 (g:mL) , salt concentration at 5% and resin content of 7%.According to these conditions, the yield of heparin was 362.02mg/kg, and under the verificative test condition the practical values was 356.65mg/kg.Salt solution-resin adsorption method was feasible and reliable, repeatability, extraction completely, more suitable for industrial production and providing the theory for the development and utilization of lungs heparin of QinBao cattle.