High-yield production of 2-keto-D-gluconate by resting cells of Gluconobacter oxydans

doi:10.13386/j.issn1002-0306.2012.19.056

High-yield production of 2-keto-D-gluconate by resting cells of Gluconobacter oxydans[J]. Science and Technology of Food Industry, 2012, (19): 177-181. DOI: 10.13386/j.issn1002-0306.2012.19.056

Citation:

High-yield production of 2-keto-D-gluconate by resting cells of Gluconobacter oxydans[J]. Science and Technology of Food Industry, 2012, (19): 177-181. DOI: 10.13386/j.issn1002-0306.2012.19.056

Citation:

High-yield production of 2-keto-D-gluconate by resting cells of Gluconobacter oxydans[J]. Science and Technology of Food Industry, 2012, (19): 177-181. DOI: 10.13386/j.issn1002-0306.2012.19.056

High-yield production of 2-keto-D-gluconate by resting cells of Gluconobacter oxydans

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Resting cells of G.oxydans DSM2003 is used to produce the 2-keto-gluco-nate, which is a primary precursor for the synthesis of D-erythoribic acid (EA) .One bacterium with high-yield production capacity was obtained by self-adapting to the high concentration of substrate, and the conversion rate had reached 24.68mmol/L/h in flask (temperature at 30℃, pH at 6.0, cell concentration at 20g/L, substrate concentration at 1100mmol/L, shaking rate at 250r/min) .While catalysis was carried in the 7L fermentor under the optimum condition, the conversion rate of 2-KGA could reach 74mmol/L/h and the cell could be reused effectively for three times.

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High-yield production of 2-keto-D-gluconate by resting cells of Gluconobacter oxydans

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