Preparation and characteristics of soy isoflavone class-specific molecularly imprint polymer microspheres based on two templates

The soy isoflavone molecularly imprint polymer microspheres was synthesized by precipitation polymerization in order to develop a new way of soy isoflavone enrichment and separation.Methods using soy isoflavone that the total contents of genistin and daidzin in soy isoflavone were 89.2 percents were used as template molecular, 4-vinyl pyridine (4-VP) were used as the functional monomer, ethylene glycol dimethacrylate (EGDMA) were used as the cross-linking agent, azodiisobutyronitrile (AIBN) were used as initiator.The characteristics of soy isoflavone class-specific molecularly imprint polymer microspheres were evaluated by means of static and dynamic adsorption experiments, also by class-specific selective experiments, structure and shape characterization research method.UV spectromertry was employed to demonstrate the mechanism of the interaction between template molecular and four kinds of functional monomers.The results showed that the 4-VP interacted powerfully with the template molecular and the interaction effect was best when the mole ratio was 1:6 between the template molecular and the 4-VP.The static adsorption experiments results showed that MIP had a good specific adsorption effect compared with NIP.The dynamic adsorption experiments results showed that the polymer microspheres would reach to saturated adsorption in 5h.The class-specific selective experiments results showed that MIP had a good class-specific selective effect and also a high absorption to various components of soy isoflavone.This molecularly imprint polymer microspheres are expected to be further research and application in the further in enriching, separating and checking the soy isoflavones.