Optimization of fermentation medium of coenzyme Q10-producing strain using response surface methodology

In order to increase the prodution of coenzyme Q10, coenzyme Q10-producing strain R-SL15 was used to obtain from the optimum fermentation medium.Plackett-Burman design and Box-Behnken response surface methodology (RSM) was used to optimize medium for coenzyme Q10 biosynthesis.According to the RSM analysis on the data of Box-Behnken design, optimal fermentation conditions for the highest coenzyme Q10 content in fermentation medium were set at glucose content of 18.90g/L, yeast extract content of 5.54g/L, (NH4) 2SO4 content of 0.98g/L、KH2PO4 content of 0.95g/L, beef extract content of 6g/L, MgSO4·7H2O content of 0.75g/L, FeSO4·7H2O content of 100mg/L, NaCl content of 10g/L and distilled water of 1L.Under the optimal conditions, coenzyme Q10 content produced by R-SL15 could reach to 51.31mg/L, which was 99.34% higher than that of non-optimized medium which was 25.74mg/L.The regression model was highly significant (R2=0.9423) and showed good fitness to the experimental data, and it could be used to predict.