Optimization of the extraction technology of protein from sloanea hemsleyana seed by response surface methodology

The extraction of protein from the sloanea hemsleyana seed by alkaline extraction and acid precipitation was studied. According to the single-factor experiments, three levels of the factors (extraction temperature, extraction time, pH and material/liquid ratio) were selected for the central composite design to establish a quadric regression equation for describing the yield of sloanea hemsleyana seed protein. By response surface analysis, the optimum extraction conditions were obtained as follows:extraction temperature of 42℃, time of 138min, pH9.6 and material/liquid ratio of 1∶25 (g/mL) , under which the highest yield of sloanea hemsleyana seed protein reached 64.97%.