DNA extraction and multiple PCR distinction research of animal food duck blood and pig blood

Objective:Study on duck blood and pig blood aimed to find a quick and easy way to extract DNA and establish multiple PCR method for identification. Methods:The KI method was used for DNA extracting from the solid massive duck blood and pig blood and was detected by PCR amplification. A multiplex PCR method was established to identify the duck, pig blood ingredients in the food of animal original and carried on the examination to animal blood products from the market. Results:The gel electrophoresis stripes indicated that this DNA extraction method was of high purity, clear and neat. And the target bands could be amplified by PCR. Multiplex PCR simultaneously amplified duck and pig bands. Conclusion:The improved DNA extraction methods could obtain the DNA of high purity. It was safe, convenient and economical compared with the traditional method. PCR. Amplification result was good, the application of multiplex PCR method could also detect duck and pig ingredients in the blood product sample.