Optimization of cultivation conditions of vibrio alginolyticus by response surface methodology

doi:10.13386/j.issn1002-0306.2012.16.022

Optimization of cultivation conditions of vibrio alginolyticus by response surface methodology[J]. Science and Technology of Food Industry, 2012, (16): 221-223. DOI: 10.13386/j.issn1002-0306.2012.16.022

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Optimization of cultivation conditions of vibrio alginolyticus by response surface methodology

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According to singe-factor experiments, three levels factors including the pH, temperature and salinity were selected to evaluate the optimum culture condition of Vibrio alginolyticus. Based on Box-Behnken central composite design principle of the Response Surface Methodology (RSM) , the OD600 was chosen as response value to determine the range of factors and levels. With the results, the optimized conditions of cultivation were as follows:pH7.62, temperature 34.55℃ and salinity 3.22%, respectively. During the germination significant interactions between pH and temperature, as well as temperature and salinity were found.

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Science and Technology of Food Industry

Optimization of cultivation conditions of vibrio alginolyticus by response surface methodology

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