Science and Technology of Food Industry

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Extraction technology of pepsin from swine by PEG/ (NH4) 2SO4 aqueous two-phase system[J]. Science and Technology of Food Industry, 2012, (15): 245-247. DOI: 10.13386/j.issn1002-0306.2012.15.082
Citation: Extraction technology of pepsin from swine by PEG/ (NH4) 2SO4 aqueous two-phase system[J]. Science and Technology of Food Industry, 2012, (15): 245-247. DOI: 10.13386/j.issn1002-0306.2012.15.082
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Extraction technology of pepsin from swine by PEG/ (NH4) 2SO4 aqueous two-phase system

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  • The pepsin was extracted and purified by PEG/ (NH4) 2SO4 aqueous two-phase system (ATPS) from stomach of swine.Extraction technology of pepsin using a newly established ATPS was investigated according to volume ratio (VR) , purification factor (PF) , specific activity (SA) , protease activity recovery yield (η) and SDS-polyacrylamide gel electrophoresis (SDS-PAGE) .The optimal ATPS conditions for pepsin partitioning was 25% PEG1000:18% (NH4) 2SO4, which activity recovery yield reached 91.5%.SDS-PAGE indicated that ATPS fraction had a major protein which molecular weight (MW) was consistent with swine pepsin.
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