Mutation breeding of Aspergillus niger 51-43 lipase with N+iron implantation and the enzyme properties

doi:10.13386/j.issn1002-0306.2012.15.060

Mutation breeding of Aspergillus niger 51-43 lipase with N+iron implantation and the enzyme properties[J]. Science and Technology of Food Industry, 2012, (15): 158-161. DOI: 10.13386/j.issn1002-0306.2012.15.060

Citation:

Mutation breeding of Aspergillus niger 51-43 lipase with N+iron implantation and the enzyme properties

Graphical Abstract

Abstract

Abstract

The Aspergillus niger 51-43 lipase was induced by N+iron implantation, for increasing the enzyme activity.The mutant L7 which was higher enzyme acticity and genetic stability was selected.The enzyme activity of this mutant from 19.28U/mL to 29.17U/mL, increased by 151.3%.The optimal pH and temperature of high-yield lipase strains L7 were 8.0 and 30℃ respectively.The lipase was highly stable in the pH range from 6.0 to 9.0 and at 50℃.Ca2+, Na+, and Mg2+ showed an advious activation to the enzyme, while Cu2+, Mn2+, Fe2+, Co2+ and Zn2+ inhibited its activity.

FullText(HTML)

References (15)

Supplements (0)

Cited By

Science and Technology of Food Industry

Mutation breeding of Aspergillus niger 51-43 lipase with N+iron implantation and the enzyme properties

Abstract

Catalog

Export File

Citation

Format

Content