Optimization of extraction technique of sulforaphane from broccoli callus via by response surface methodology (RSM)

doi:10.13386/j.issn1002-0306.2012.15.033

Optimization of extraction technique of sulforaphane from broccoli callus via by response surface methodology (RSM)[J]. Science and Technology of Food Industry, 2012, (15): 206-209. DOI: 10.13386/j.issn1002-0306.2012.15.033

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Optimization of extraction technique of sulforaphane from broccoli callus via by response surface methodology (RSM)

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Using broccoli callus as experimental material, based on single factor tests, through Box-Benhnken central combination design and RSM were obtained the optimum extraction conditions of sulforaphane.The results showed that the optimum extraction conditions were the extraction temperature 25.7℃, the extraction time 2.8h, the liquid solid ratio 1:30 (g/mL) , the extraction times 3.The test value was 0.0067% under the above conditions (theory value was 0.0078%) , that optimize the use of response surface method of the extraction conditions were reliable.

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Science and Technology of Food Industry

Optimization of extraction technique of sulforaphane from broccoli callus via by response surface methodology (RSM)

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