Optimization of fermentation process on a fibrinolytic enzyme from Enterococcus faecalis EF608

doi:10.13386/j.issn1002-0306.2012.15.031

Optimization of fermentation process on a fibrinolytic enzyme from Enterococcus faecalis EF608[J]. Science and Technology of Food Industry, 2012, (15): 165-168. DOI: 10.13386/j.issn1002-0306.2012.15.031

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Optimization of fermentation process on a fibrinolytic enzyme from Enterococcus faecalis EF608

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The optimum fermentation conditions for the fibrinolysin from Enterococcus faecalis EF608 were investigated.The results showed that the optimal fermentation condition were as the following:2% glucose, 0.75% peptone, 0.75% beef extract, 0.5% yeast extract, 1% K2HPO4·3H2O, 0.2% NaCl, 0.02% MgSO4·7H2O, initial pH7.5, temperature 37℃, 15% of medium volume and 6% of inoculums, incubation time for 10h.Under these conditions, the fibrinolytic enzyme activity of the fermented broth have reached 1833IU/mL.

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Science and Technology of Food Industry

Optimization of fermentation process on a fibrinolytic enzyme from Enterococcus faecalis EF608

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