Ultrasonic extraction of polysaccharides from Matteuccia struthiopteris and their DPPH free radical scavenging activity

The condition for ultrasonic extraction of polysaccharides from Matteuccia struthiopteris were optimized by one-factor-at-a-time and orthogonal array design methods.Polysaccharide from Matteuccia struthiopteris was tested with DPPH· to estimate their antioxidative activity.The results showed that the best extraction conditions were determined as below:ethanol liquid ratio was 1:50 (g/mL) , extraction temperature was 80℃, ultrasonic power was 150W, ultrasonic time was 30min.The extraction yield of polysaccharide in rhizome, trophophyll and sporophyl was 9.67%, 3.30% and 3.43% respectively.The polysaccharide had high antioxidative activity, revealing an IC50 of 68.68μg/mL.Investigating ultrasonic traction of polysaccharides from matteuccia struthiopteris and their DPPH free radical scavenging activity, it is a great direction significance in the development and utilization of matteuccia struthiopteris.