Determination of organic acids in Schisandra chinensis by HPLC

The main organic acids of Schisandra chinensis were determinate by high performance liquid chromatography, choosing cation exchange column Shim-pack SCR.102H (8.0mm i.d.×300mm) as analytical column of Schisandra chinensis organic acids compared with Agilent ZOBAX×SB-C18 (250mm×4.6mm, 5μm) column.Mobile phase was 0.1% H3PO4 with flow rate of 0.8mL/min at 45℃.Detection wavelength was 210nm.The injection volume was 10μL.The main organic acids of Schisandra fresh fruit are malic acid, citric acid and shikimic acid which was first found in Schisandra chinensis, and the citric was (3.26±0.06) g/100g, malic acid was (1.13±0.04) g/100g, shikimic acid was (0.53±0.01) g/100g.