Comparison of two methods in preparation of enzyme conjugates and optimization of Listeria monocytogenes TAS-ELISA KIT

To develop a low-cost, rapid and accurate TAS-ELISA KIT.This study preparated AP-IgG and HRP-IgG by glutaraldehyde method and improved periodate method respectively and compared two enzyme conjugates of Listeria monocytogenes TAS-ELISA KIT, further investigated some indexes including the sensitivity, specificity, stability, shelf life.The results showed that HRP-IgG preparated with improved periodate method was more effective, the molar ratios was 2.9, the rate of enzyme-labeled was 27%.The detection time of KIT was 6h, the detection cost was 3.5/well.Specific experiment, interference experiment, repetitive experiment and stable experiment indicated that the HRP-IgG preparated with improved periodate method was specific and reliable.The performance of self-made TAS-ELISA KIT could reach commercial KIT, it also could provide theoretical basis for industrialization of the product.