Abstract: Objective: To study the effect of low GI multigrain cocoa powder (C-MGP) on blood glucose in diabetic mice. Methods: Fifty-two male SPF mice were randomly divided into 6 groups, including normal control group, model group, metformin group, flushing powder low, medium and high-dose groups (5%, 10%, 30% C-MGP). Mice in normal control group were fed basal diet, mice in model group and metformin group were fed high-glucose and high-fat diets. Mice in low dose group (5% C-MGP), medium dose group (10% C-MGP) and high dose group (30% C-MGP) were fed high-glucose and high-fat diets containing 5% (mass fraction), 10% (mass fraction) and 30% (mass fraction) low GI mixed grain cocoa, respectively. After four weeks of high-fat diet, type Ⅱ diabetic mouse model was established by intraperitoneal injection of streptozotocin (STZ) (30 mg/kg). After successful modeling, feeding was continued for 4 weeks, basic indexes and glucose and lipid metabolism indexes were measured during the experiment in mice. Results: After high-fat diet combined with STZ induction, compared with the model group, 10% C-MGP and 30% C-MGP had significantly lower food intake and water intake (P<0.01), and significantly higher body weight (P<0.01). Compared with the model group, 30% C-MGP significantly decreased liver and kidney indexes, fasting blood glucose (FBG), glycosylated hemoglobin (GHb), fasting insulin (FINS) and insulin resistance index (HOMA-IR), and improved glucose tolerance (GT) in STZ-induced diabetic mice. Total glyceride (TG), total cholesterol (TC), low density lipid-cholesterol (LDL-C), high density lipid-cholesterol (HDL-C) and free fatty acids (FFA) levels were improved, hepatic glycogen (HG) synthesis was significantly promoted (P<0.01), and liver fat accumulation was improved. Significantly increased the content of serum glucagon like peptide-1 (GLP-1) (P<0.01), significantly decreased the level of serum lipopolysaccharide (LPS) (P<0.01). Conclusion: The 30% C-MGP had the effects of regulating glucose and lipid metabolism, and reduces diabetes symptoms on SZT induced diabetic mice, which is valuable for further development and utilization.