Abstract: To investigate the quality difference of Auricularia auricular-judaefrom different producing areas, Auricularia auricular-judaefor sale from 15 producing areas were used to tested materials in this study, 17 amino acid contents in Auricularia auricular-judae were used to assessing index, which were analyzed by principal component analysis and clustering analysis. Results showed that 4 principal components were extracted from 17 assessing index, their accumulative variance comtribution rate reached 89.674%, and could reflect preferably comprehensive information of Auricularia auricular-judae from 15 producing areas. Phe, Arg, Pro, Met and Ser contents could be comprehensive assessing index of Auricularia auricular-judaefor sale from 15 producing areas. The order of Auricularia auricular-judaequality from good to bad based on amino acid content of Auricularia auricular-judae for sale from 15 producing areas by subordinate fuction method was Heilongjiang>Liaoning>Xizang>Neimenggu>Shanxi>Jiangxi>Zhejiang>Hebei>Jilin>Xinjiang>Guizhou>Yunnan>Hubei>Gansu>Sichuan. Auricularia auricular-judaefor sale from 15 producing areas were divided into three classes by clustering analysis. Class I with higher comprehensive scores included 3 producing areas of Heilongjiang, Liaoning and Xizang. Class II with medium comprehensive scores included 11 producing areas of Neimenggu, Shanxi, Jiangxi, Zhejiang, Hebei, Jilin, Xinjiang, Guizhou, Yunnan, Hubei and Gansu, Class III with lower comprehensive scores included 1 producing area of Sichuan.