Abstract: In this study,the effect of alkali-enzyme combined with dehairing methods on the nutritional composition and quality of cowhide were studied. Changes in nutritional composition were evaluated by analysis of moisture,protein,fat,fatty acid,amino acid composition,trace elements and heavy metals,and changes in quality were evaluated by indicators such as pH,CIELAB,WHC(water hold capacity),cooking loss,shear force and texture. The results showed that alkali and enzyme had a certain degree of hydrolysis of proteins and amino acids in cow leather. After alkaline-enzymatic combined dehairing,the protein content of cowhide was decreased by 4.89%,and the contents of 16 kinds of amino acids was reduced to varying degrees,the main saturated fatty acid cardamom(C14:0) and stearic acid(C18:0) in cowhide,after dehairing the contents was decreased by 8.65% and 0.71%,respectively,the palmitic acid(C16:0) was increased by 5.12%,the contents of monounsaturated fatty acids was increased by 12.89%,and the polyunsaturated fatty acids was decreased by 7.53%. After alkali-enzyme combined dehairing,the cooking loss was increased from 5% to 30%,the water holding capacity was reduced by 25%,the shear force value was reduced by 42%,and the hardness,the gumminess,the chewiness the cohesiveness and adhesiveness were reduced to varying degrees,cowhide tenderness had improved. The results indicated that after alkali-enzyme combined dehairing,the nutrients of cowhide changed to different degrees,and the tenderness was improved.