Abstract: Objective:To study the effect of Tremella aurantialba lipid extracts on the penetration of Evans Blue through the blood brain barrier. Methods:Three groups of experiments were carried out on the Tremella aurantialba lipid extracts:The effect of the mode of administration on its penetration,the effect of the dose on its penetration,and the comparison of the effects of Tremella aurantialba lipid extracts with borneol. Formamide-spectrophotometry was used to detect the concentration of Evans Blue in tissues,and the degree of penetration through blood brain barrier was determined according to the concentration of Evans Blue in brain tissues. Results:The mixed administration method was better. The concentration of Evans Blue in brain tissue was the highest at 13.25 μg/g. The effect of tail vein injection was better,the concentration of Evans Blue in brain tissue was the highest at 15.96 μg/g. The high-dose group had better effect of administration. The concentration of Evans Blue in the brain was higher than that in the control group at different time points. And the concentration of Evans Blue was the highest in the brain tissue at 2 h,but the concentration was lower in the liver and spleen tissues,which indicated that the optimal time of drug in the brain was 2 h. The comparison of Tremella aurantialba lipid extracts with borneol showed that the concentration of the Tremella aurantialba lipid extracts was the highest at 2 h,which was higher than that of the borneol group,and there was a significant difference compared with the control group. Conclusion:The Tremella aurantialba lipid extracts had the effect to promote Evans Blue through the blood brain barrier with great research value.