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中国精品科技期刊2020
彭青枝, 杨冰洁, 王璐荣, 杨书珍, 朱晓玲. 3种杀菌处理对炭黑曲霉生长和赭曲霉毒素A产生量的影响[J]. 食品工业科技, 2019, 40(10): 152-157. DOI: 10.13386/j.issn1002-0306.2019.10.025
引用本文: 彭青枝, 杨冰洁, 王璐荣, 杨书珍, 朱晓玲. 3种杀菌处理对炭黑曲霉生长和赭曲霉毒素A产生量的影响[J]. 食品工业科技, 2019, 40(10): 152-157. DOI: 10.13386/j.issn1002-0306.2019.10.025
PENG Qing-zhi, YANG Bing-jie, WANG Lu-Rong, YANG Shu-zhen, ZHU Xiao-ling. Effects of Three Fungicidal Treatments on Growth of Aspergillus carbonarius and Production of Ochratoxin A[J]. Science and Technology of Food Industry, 2019, 40(10): 152-157. DOI: 10.13386/j.issn1002-0306.2019.10.025
Citation: PENG Qing-zhi, YANG Bing-jie, WANG Lu-Rong, YANG Shu-zhen, ZHU Xiao-ling. Effects of Three Fungicidal Treatments on Growth of Aspergillus carbonarius and Production of Ochratoxin A[J]. Science and Technology of Food Industry, 2019, 40(10): 152-157. DOI: 10.13386/j.issn1002-0306.2019.10.025

3种杀菌处理对炭黑曲霉生长和赭曲霉毒素A产生量的影响

Effects of Three Fungicidal Treatments on Growth of Aspergillus carbonarius and Production of Ochratoxin A

  • 摘要: 研究了3种常见的杀菌处理:紫外照射处理、温度处理和杀菌剂处理(抑霉唑、多菌灵和咪鲜胺)对果蔬采后主要致病和产毒菌——炭黑曲霉的生长及赭曲霉毒素A(Ochratoxin A,OTA)产生量的影响。结果表明:紫外照射处理可以有效降低孢子萌发和菌丝生长,但OTA产生量却随着紫外照射时间延长呈先增加后减少的趋势,其中处理45 min时OTA产生量是对照组的3.18倍;温度处理对炭黑曲霉的生长和OTA的产生影响表现一致,4 ℃下可以完全抑制生长和OTA的产生,25 ℃时,其生长量和毒素产生量最多;常用采后化学杀菌剂多菌灵、抑霉唑和咪鲜胺对炭黑曲霉孢子萌发和菌丝生长均表现出显著的抑制作用;但中等剂量的化学杀菌剂处理(多菌灵,0.12 μg/mL;抑霉唑和咪鲜胺,0.06 μg/mL)会刺激炭黑曲霉产生大量的OTA,产生量分别是对照组的6.31倍、23.7倍和6.17倍。因此,紫外照射杀菌处理时间不足和化学杀菌剂使用浓度较低时均会刺激炭黑曲霉产生大量的OTA,正确使用杀菌处理条件对于保证果蔬采后安全贮藏与加工具有重要意义。

     

    Abstract: The effects of postharvest antifungal treatments including UV irradiation,temperature treatment and fungicide treatment(imazalil,prochloraz and carbendazim)on the growth of A. carbonarius and production of ochratoxin A(OTA)were evaluated. The results showed that UV irradiation significantly decreased the spore germination and mycelial growth of A. carbonarius. However,OTA production were increased with the prolong of the pathogen exposed to UV irradiation,and OTA production was up to 3.18 times compared with control,when the exposed time was 45 min. Effects of Temperature treatment on the growth of the pathogen and OTA production were similar,which could be inhibited completely at 4 ℃and improved at 25 ℃. Fungicide treatment decreased the spore germination and mycelial growth obviousl. However,Fungicide treatment with a medium dose(carbendazim,0.12 μg/mL;imazalil and prochloraz,0.06 μg/mL)could stimulate the pathogen producing a great deal of mycotoxin,and OTA production were 6.31,23.7 and 6.17 times separately of control. Therefore,UV irradiation and chemical fungicidal treatment under improper conditions could stimulate A. carbonarius producting a lot of OTA,and reasonable using fungicidal treatment have great significance for ensuring the safe storage,sales,and processing of grapes.

     

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