Abstract: In order to simplify the experimental steps of histamine determination in GB 5009.208-2016 and improve the accuracy and precision of the experiment, the paper ameliorated the method on the basis of the Chinese national standard. The results showed that, changed the solvent of P-nitroaniline solution to concentrated hydrochloric acid, and selected 60℃ to keep warm for 0.5 h instead of room temperature soaking. The samples were extracted by vortex oscillating. And the extraction times of hydrochloric acid were changed to 2 times on this basis. The extraction solution of hydrochloric acid was changed to 1.0 mL. The correlation coefficient of the improved linear equation was 0.9991.The recovery rate of the improved method were 92.90%~94.70%, 91.50%~94.10% and 100.20%~102.20%, and the RSD was 1.84%~2.32%. The method was simple and rapid. It could be used for the determination of histamine in fish better.