Abstract: Objective:To evaluate the antioxidant and antibacterial activity of two kinds of polyporaceae mushrooms (Coriolus versicolor and Trametes robiniophila), and investigate chemical constituents of their low polarity part. Methods:Extracting the powder of two mushrooms by soxhlet method. The above extracts were sequentially extracted with petroleum ether, ethyl acetate, n-butanol and distilled water. The antioxidant and antibacterial activity of different solvent extracts were investigated by the DPPH free radical clearance method and agar diffusion plate method. Finally, the petroleum ether extracts of the two mushrooms were methylation and analyzed by GC-MS. Results:The antioxidant activity of each solvent extract of the two mushrooms showed clear dose-effect relationship, and the ethyl acetate and n-butanol extract from Coriolus versicolor had stronger antioxidant activity. However, there was no obvious antibacterial effect of these two mushrooms. The results of GC-MS analysis showed that there were 27 compounds in petroleum ether extraction part of the Coriolus versicolor, and 39 compounds in Trametes robiniophila. Methyl linoleate, 1, 2-benzenedicarboxylic acid, methyl 2-hydroxy-tetracosanoate, palmitic acid and ergosta-14, 22-dien-3-ol, (3β, 5α, 22E) had a high contents in two mushrooms. Conclusion:This study had clarified part of the chemical constituents of these two mushrooms, and their antibacterial and antioxidation activity had been studied. It would provide some theoretical basis for their further development and utilization in anti-aging drugs and health food.