Abstract: In this study,there were three stocking densities of Penaeus monodon selected(100,200,300 tail/m²).In order to study the effect of stocking density on muscle quality of Penaeus monodon,the basic nutrients and water holding capacity were determined,and the differences of the flavour were analyzed by electronic nose and electronic tongue,combined with sensory quality and texture characteristics. The results showed that with stocking density increasing,the contents of moisture and ash increased,the contents of crude protein and total sugar decreased,the water holding capacity decreased extremely significantly(p<0.01).Sensory evaluation and electronic tongue analysis showed that the main difference of Penaeus monodon of three stocking densities was umami and M3 had the worst umami,electronic nose analysis showed that the main difference of Penaeus monodon of three stocking densities could be distinguished by the four sensors(No.6,No.7,No.8,No.9)and the four substances were also the main volatile odors of Penaeus monodon muscle,sensory evaluation and texture analysis showed that the hardness,resilience,cohesion and chewiness all decreased. From a comprehensive assessment of the economic and muscle quality,200 tail/m² should be the recommended stocking density in actual production.