Abstract: In order to optimize the preparation technology of protein powder from porcine intestinal mucosa by protease,the type of protease was screened and the enzymatic hydrolysis conditions was optimized according to soluble protein content inhydrolysates by single factor and response surface methodology.Results showed that the papain enzymolysis intestines mucous membrane protein powder preparation soluble protein content reaches 7.37%±0.06%,significantly higher than that of trypsin and pepsin enzyme hydrolysis of intestinal mucous membrane protein powder, soluble protein content.The best enzyme hydrolysis conditions for:hydrolysis time 6.5 h,the adding amount of 5400 U/g protein,liquid-solid ratio 4 mL/g,under the condition of the enzyme solution of soluble protein content in the product was 15.81%±0.04%.These results indicated that the enzymatic preparation of intestinal mucous membrane protein powder is reasonable and feasible,and provided theoretical basis and reference for the development and utilization of animal by-products.