小红豆抗菌蛋白的分离纯化及其功能活性
Isolation and purification of an antifungal protein from small red bean and its functional properties
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摘要: 研究旨在从小红豆中分离纯化得到一种具有抗菌活性的蛋白,对其部分生物活性进行表征和研究。通过缓冲液抽提（NaAc-HAc,20 mmol/L,pH5.4）、硫酸铵分级沉降、Affi-gel blue gel亲和色谱、Sephadex SP C-25离子交换色谱及Sephadex G-50凝胶过滤色谱进行分离纯化,经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析鉴定其相对分子质量,并对其抗真菌活性进行表征。结果表明,从小红豆中分离纯化出的一种抗菌蛋白,经SDS-PAGE鉴定达到电泳纯,且其相对分子质量约为4 kDa,通过纯化的抗菌蛋白对来自茄子、甜瓜及苦瓜的尖孢镰刀菌的抑制实验,证明此抗菌蛋白具有较强的抗真菌活性。从小红豆中分离纯化出的一种分子质量约为4 kDa的抗菌蛋白,其对真菌具有抑制作用,且其抑制率对浓度的依赖性较高。Abstract: This essay was aim to isolate, purificate and partial characterize the proteins with antifungal activity from the small red beans. A protein with antifungal activity was isolated and purified from small red beans by the procedure containing extraction, ammonium sulfate precipitation, Affi-gel blue gel chromatography, ion-exchange chromatography, and gel filtration chromatography.SDS-PAGE was used to analysis its purity and molecular weight.The result showed that the antifungal protein isolated and purified from small red beans exhibited a molecular mass of 4 kDa in SDS-polyacrylamide gel electrophoresis.Antifungal activity experiment showed that the protein had a strong inhibitory activity towards the growth of Fusarium oxysporum from eggplant, muskmelon and bitter gourd.An antifungal protein with a molecular mass of 4 kDa was isolated from small red beans.It exerted inhibitory activity on Fusarium oxysporum and had a high dependence on the concentration of protein.
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