正交实验法优化酒石酸提取黑豆皮花色苷工艺

Optimization of tartaric acid extraction of anthocyanins from soybean hulls by orthogonal experiment

摘要: 目的:优化酒石酸提取黑豆皮花色苷的工艺。方法:以黑豆皮为原料,黑豆皮花色苷含量为指标,在单因素实验基础上,采用正交实验设计法优化酒石酸提取黑豆皮花色苷的工艺。结果:酒石酸提取黑豆皮花色苷最佳提取工艺为酒石酸质量分数40%,料液比1∶30 g/m L,加热温度60℃,加热时间150 min。花色苷含量14.32 mg/g。优选工艺条件下,黑豆皮花色苷含量14.32 mg/g。结论:结果稳定,具有生产应用价值。

Abstract: Objective: Optimization of tartaric acid extraction of anthocyanin from black soybean hulls. Methods: Black soybean hulls and black soybean anthocyanin content were used as raw materials and the index, the process optimization of tartaric acid extraction of black soybean anthocyanin were investigated by orthogonal experimental design. Results: The best extraction process of tartaric acid extraction of anthocyanins from black soybean hulls was the tartaric acid concentration of 40%, solidliquid ratio 1∶ 30 g/m L, extraction temperature of 60 ℃, extraction time of 150 min.According to the optimal process conditions of black soybean hulls, anthocyanin yield was 14.32 mg/g. Conclusion: The result of the experiment proves that the result is stable and has the value of production and application.