Abstract: In the present study, laccase gene c DNA was cloned and sequenced from‘shixia'longan cultivar using RT-PCR and RACE, which was named as Dl Lac. The NCBI numbers were KY051551. The Dl Lac gene consists of 1898 bp encoding a polypeptide of 576 amino acids.Dl Lac gene had the highest homology with the Litchi chinensis ( 94%) through the NCBI blastn.Amino acids of laccase gene sequence had 3 highly conservative domain structure: Cu-oxidase-3, Cu-oxidase and Cu-oxidase-2.During the normal and cold storage, combining the relationship of pericarp browning and Dl Lac expression, it suggested that the laccase gene in pericarp may contribute to the postharvest longan pericarp browning.