Abstract: In the study, the adsorption of skimmed milk at Au electrode was nvestigated. Differential capacitance were used to study skimmed milk of different concentrations dispersion adsorption property on the surface of Au electrode, the adsorption behavior of milk on the Au electrode was characterized by cyclic voltammetry and electrochemical impedance spectroscopy.The adsorption behavior of different skimmed milk dispersion on gold crystal surface were researched by QCM. The results showed that within a certain concentration range, skimmed milk dispersion was monomolecular layer adsorption on the surface of the Au electrodes and in line with the Langmuir adsorption isotherm equation. Skimmed milk on the adsorption of gold crystal surface gradually reached saturation with the increasing skimmed milk concentration. In different p H buffer solutions, the adsorption quantity of milk on gold crystal surface was different, with the maximum adsorption in the p H of 5. At the same concentration and p H, adsorption capacity increased firstly and then decreased with the increase of ionic strength.